Genetic Variation Analysis of CYP2R1 Gene Sequence in Homo Sapiens (PopSet: 1928007955) Using RFLP In Silico

Abstract

The CYP2R1 gene is the gene responsible for Vitamin D metabolism. Plays a crucial role in maintaining bone health, the immune system, and various other biological functions in Homo sapiens. The CYP2R1 gene resides on chromosome 11p15.2 and has five exons, spread over a region of about 15.5 kb. In-silico is an approach to research approach whose use will be discussed with advances in technology and available databases. This approach is very commonly used in the medical and healthcare field. The method used is to use the NCBI website, screen the restriction enzymes within the silico web and continue with the benchling website, restriction enzyme within the silico web and continue with the benchling site. The results obtained using the MseI enzyme which produces two allele fragments, namely Allele A1 and Allele A2. Allele A1 has fragments measuring 100 and 120 bp and allele A2 has a fragment size of 123 bp. Allele A1 is more dominant than allele A2 because it has a presentation of 93.9%.