Optimization of Carbon Source and Organic Nitrogen for the the growth of Andalas Plant Endophytic Bacteria (Morus macroura Miq.) Isolate ATB 10-6 and Production of Antifungal Compounds

Abstract

Isolate ATB 10-6 is an endophytic bacteria of Andalas plant which is known to be able to produce acrive antifungal compounds, especially against fungi from the genus Candida. The production of active compounds by endophytic bacteria can be done through a fermentation process. One of the factors that influence fermentation is the medium. Every bacteria has different nutritional needs. The purpose of this study was to determine th growth of endophytic bacteria in Andalas plant isolate ATB 10-6 and the production of antifungal compounds.  The design used in this study was a factorial completely randomized design. The types of carbon used in the optimization process are maltose, glucose, lactose, starch and sucrose. Furthermore, the types of organic nitrogen used are yeast extract, malt extract, beef extract and pepton water. The concentration of the carbon source used was 0,5% and the concentration of the organic nitrogen source was 1%. Bacterial growth was observed by measuring optical density (OD) while the antifungal activity was carried out by diffusion method. The result showed that for the growth of endophytic bacteria Andalas starch was the best carbon source and yeast extract as the best source of organic nitrogen. Furthermore, for the production of antifungal compounds by endophytic bacteria Andalas maltose and starch are the best sources of carbon and yeast extract as the best sources of organic nitrogen. The interaction of these two nutrients show that starch and yeast extract are the best sources of nutrients for the production of antifungal compounds by the endophtic bacteria Andalas.